定量盘法检测菌落总数®

令装瓶厂和生产厂更方便地检测菌落总数

 
Bottled Water Recreational Water
 
 

简化异养微生物的检测和量化

  • 无需高压灭菌、过滤或配置培养基
  • 无需菌落计数或解释
 
定量盘检测菌落总数
 

致电 400 678 6682、021-61279528、010-84423000或发送邮件到 water@idexx.com

定量盘检测菌落总数:提供可信量化结果

 

 
  • 便捷的 100-mL 样本量 — 检测源水、交通工具用水、生产线用水和成品。
  • 程序简单无需特别培训。
  • 与 科立得®、Enterolert® 和 Pseudalert® 检测产品共享同样的定量盘® 系统,使效率最大化。
  • 结果 44–72 小时有效。
产品信息

样品体积:100 mL

检测时间:44–72 个小时

保质期:2–25°C 下可保存 12 个月;保存时避免阳光直射

读取结果:样品显荧光表明存在异养生物

使用方法:使用 IDEXX 51孔定量盘®97孔定量盘® 进行定量检测

 

试剂配51孔定量盘试剂盒(用于 100-mL 水样):

  • 20 次检测包装 #WHQT-20
  • 200 次检测包装 #WHQT-200
 

试剂配97孔定量盘试剂盒(用于 100-mL 水样):

  • 20 次检测包装 #WHQT2K-20
  • 200 次检测包装 #WHQT2K-200

定量盘® 检测菌落总数工作原理

定量盘® 检测菌落总数用于定量检测 100 mL 水中的异养微生物数量。

 

该检测产品采用 IDEXX 专利的复合酶底物技术™,通过定性检测相关微生物中已知存在的主要酶,来检测可能存在的异养微生物。 该检测产品采用的复合酶底物可在被介水传播的细菌分解代谢时产生蓝色荧光。

 

将样品/试剂混合液加入定量盘®,培养后检查荧光孔。 荧光孔数量相当于原始样品中的总异养微生物的最大可能数 (MPN)。

 

定量盘检测菌落总数可在 44–72 小时培养后检测微生物 1 cfu/100 mL。

 

 

View/Print HPC for Quanti-Tray Procedure

 

使用定量盘检查菌落总数的分步指南

 
 
 
 

第 1 步

在水样中加入试剂(水样会变成粉红色)。

 
   
 

第 2 步

加入到51孔定量盘®97孔定量盘®

   
 

第 3 步

使用程控定量®封口机封口;培养 44 小时。

   
 

第 4 步

在紫外灯下读取结果;数出蓝色荧光孔的数目,并参照 MPN 表。

 

常见问题解答

 

Which water types can be tested?

The HPC for Quanti-Tray® Test is designed specifically for bottled-water samples. Bottled-water samples are defined as samples taken from any point in the bottled-water manufacturing process (from the original water source, to transportation vehicles, to the manufacturing line, to the finished product).

 

What is the sample incubation time and temperature?

Water samples are incubated at 36ºC (±2ºC) for 44 hours, and results are valid up to 72 hours.

 

What is the detection limit?

The HPC for Quanti-Tray Test detects heterotrophic organisms at 1 cfu/100 mL.

 

Does the test include a comparator?

There is no comparator for the HPC for Quanti-Tray Test. Use a negative control for comparison when you interpret results.

 

Are results valid prior to 44 hours?

Yes, if the results are positive. An inoculated HPC for Quanti-Tray Test sample that is positive before 44 hours is a confirmed positive test. However, for an accurate quantitative determination of heterotrophic count in a sample, the results should be read between 44 and 72 hours of incubation.

 

Are results valid after 72 hours?

Yes, if the results are negative. If an inoculated HPC for Quanti-Tray Test sample is inadvertently incubated over 72 hours, lack of fluorescence is a valid negative test. However, a fluorescent read after the 72 hours is not valid and should be repeated or verified.

 

Can either a 51-well or a 97-well Quanti-Tray® be used?

Yes, both the Quanti-Tray® and Quanti-Tray®/2000 can be used with the HPC for Quanti-Tray Test in the 100-mL format.

 

What should be the color and consistency of the test powder?

The HPC for Quanti-Tray Test reagent powder should be pink in color and of a free-flowing, granular consistency. Direct any concerns about the color or integrity of the powder to IDEXX Technical Service.

 

How should customers dispose of a positive HPC sample?

Check with your local, state and/or federal authorities for proper disposal of bacteriological biohazard materials at your facility.

 

Can HPC for Quanti-Tray be used with carbonated water samples?

HPC for Quanti-Tray should not be used with carbonated water samples.

 

定量盘检测菌落总数:您需要的产品,为您提供可信的结果

 

注意:可用性因国家而异。

 
 

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